Well it depends if it's the drive you expect the main PC to boot off. If it is, then I wouldn't expect it to work. You'll probably have to re-install windows. Also, if it was the drive the old PC booted off, then it will of course have problems if it can't find it.
Check to see if it comes up in BIOS when you start the PC - you know, hold down F1 or DEL or whatever it says to go into setup mode right at the start when you boot up.
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